These cloning cylinders facilitate the isolation of individual cell clones, each generated from a single cell, yielding a homogenous population of cells. To isolate a clone, a thin layer of grease is applied to the bottom edge of the cylinder and then inverted over the clone of choice. After adding a small volume of Trypsin or EDTA, the dish is incubated at 37�C (98.6�F) briefly until cells detach. Cells are then collected from inside the cylinder and transferred to another vessel for further growth.
Polystyrene cloning cylinders are supplied in sterile packs. Three sizes: 4.7mm I.D. x 8mm H, 6.4mm I.D. x 8mm H and 9.5mm I.D. x 11mm H. Each cylinder is tapered so the bottom edge is wider than the top ensuring a good sealing surface. Packed 10 per bag, one size.